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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
08/03/2023 |
Actualizado : |
08/03/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
COLOMBATTI OLIVIERI, M. A.; FRESIA, P.; GRAÑA, M.; CUERDA, M.X.; NAGEL, A.; ALVARADO PINEDO, F.; ROMANO, M. I.; CAIMI, K.; BERNÁ, L.; SANTANGELO, M. P. |
Afiliación : |
M.A. COLOMBATTI OLIVIERI, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolas ´ Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; M. GRAÑA, Unidad de Bioinformatica, Institut Pasteur de Montevideo, Mataojo 2020, CP11400, Montevideo, Uruguay; M. X. CUERDA, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; A. NAGEL, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; F. ALVARADO PINEDO, Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE), Facultad de Ciencias Veterinarias - Universidad de La Plata (UNLP), Buenos Aires, Chascomus, Argentina; M. I. ROMANO, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina; K. CAIMI, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina; L. BERNÁ, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, CP, Uruguay; M. P. SANTANGELO, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina. |
Título : |
Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype. |
Complemento del título : |
Letter. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Tuberculosis, 2023, volume 138, article 102299. doi: https://doi.org/10.1016/j.tube.2022.102299 |
ISSN : |
1472-9792 |
DOI : |
10.1016/j.tube.2022.102299 |
Idioma : |
Inglés |
Notas : |
Article history: Received 30 September 2022; Received in revised form 28 November 2022; Accepted 19 December 2022; Available online 21 December 2022. -- Correspondence authors: Berná, L.; Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, CP, Uruguay, email:lberna@pasteur.edu.uy ; Santangelo, M.P.; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina, email:santangelo.maria@inta.gob.ar -- FUNDING: This work was supported by the National Institute of Agriculture Research (grant number PNSA PDI105); National Agency for Science and Technology Promotion, Argentina (grant number PICT 2016-0287); CONICET-Institut Pasteur Bilateral Cooperation Program. -- |
Contenido : |
In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. © 2022 Elsevier Ltd |
Palabras claves : |
Genomics; Oxidative stress; Phenotype; Virulence; WGS. |
Asunto categoría : |
A50 Investigación agraria |
Marc : |
LEADER 03150naa a2200325 a 4500 001 1063970 005 2023-03-08 008 2023 bl uuuu u00u1 u #d 022 $a1472-9792 024 7 $a10.1016/j.tube.2022.102299$2DOI 100 1 $aCOLOMBATTI OLIVIERI, M. A. 245 $aGenomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 30 September 2022; Received in revised form 28 November 2022; Accepted 19 December 2022; Available online 21 December 2022. -- Correspondence authors: Berná, L.; Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, CP, Uruguay, email:lberna@pasteur.edu.uy ; Santangelo, M.P.; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina, email:santangelo.maria@inta.gob.ar -- FUNDING: This work was supported by the National Institute of Agriculture Research (grant number PNSA PDI105); National Agency for Science and Technology Promotion, Argentina (grant number PICT 2016-0287); CONICET-Institut Pasteur Bilateral Cooperation Program. -- 520 $aIn a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. © 2022 Elsevier Ltd 653 $aGenomics 653 $aOxidative stress 653 $aPhenotype 653 $aVirulence 653 $aWGS 700 1 $aFRESIA, P. 700 1 $aGRAÑA, M. 700 1 $aCUERDA, M.X. 700 1 $aNAGEL, A. 700 1 $aALVARADO PINEDO, F. 700 1 $aROMANO, M. I. 700 1 $aCAIMI, K. 700 1 $aBERNÁ, L. 700 1 $aSANTANGELO, M. P. 773 $tTuberculosis, 2023, volume 138, article 102299. doi: https://doi.org/10.1016/j.tube.2022.102299
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
15/10/2014 |
Actualizado : |
31/08/2017 |
Tipo de producción científica : |
Presentaciones Orales |
Autor : |
QUINCKE, A.; MORON, A.; DIAZ, R.; SAWCHIK, J.; TERRA, J. |
Afiliación : |
JUAN ANDRES QUINCKE WALDEN, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DAVID ALEJANDRO MORON YACOEL, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ROBERTO MIGUEL DIAZ ROSSELLO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; JORGE SAWCHIK PINTOS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; JOSE ALFREDO TERRA FERNANDEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Experimentos de largo plazo con rotaciones agrícola-ganaderas en Uruguay. |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
In: CONGRESO ARGENTINO DE PRODUCCIÓN ANIMAL, 34.; JOINT MEETING AAPA-ASAS, 1., 2011, Mar del Plata. Presentaciones. Mar del Plata: ASAS/AAPA, 2011. |
Idioma : |
Español |
Thesagro : |
ROTACIONES AGRÍCOLA-GANADERAS. |
Asunto categoría : |
-- |
Marc : |
LEADER 00580nam a2200157 a 4500 001 1051079 005 2017-08-31 008 2011 bl uuuu u00u1 u #d 100 1 $aQUINCKE, A. 245 $aExperimentos de largo plazo con rotaciones agrícola-ganaderas en Uruguay.$h[electronic resource] 260 $aIn: CONGRESO ARGENTINO DE PRODUCCIÓN ANIMAL, 34.; JOINT MEETING AAPA-ASAS, 1., 2011, Mar del Plata. Presentaciones. Mar del Plata: ASAS/AAPA$c2011 650 $aROTACIONES AGRÍCOLA-GANADERAS 700 1 $aMORON, A. 700 1 $aDIAZ, R. 700 1 $aSAWCHIK, J. 700 1 $aTERRA, J.
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